Vacuum Filtrations
Vacuum filtration is a laboratory equipment that allows you to separate liquids from solids. The membrane filtration technique allows samples to be passed through a membrane filter using the vacuum filtration. Contaminants in the sample will be filtered and concentrated on the surface of the membrane.
Corning outlines 3 easy steps on how to choose a vacuum filter. First, you need to choose your pore size based on your application needs. There are 3 types of needs which are as below:
- Clarification or pre-filtration of buffers (0.45 µm pore size)
- Sterile filtration of media and reagents (0.2/0.22 µm pore size)
- Mycroplasma removal (0.1 µm pore size).
Next, optimize the flow rate by picking a suitable membrane based on your application. Membrane filter act as a barrier to separate any bacteria, particulates or contaminants from water which can impart the water color, odour nor tastes. It is a thin layer of material such as Polyethersulfone (PES), Cellulose Acetate (CA), Cellulose Nitrate (CN), MCE or nylon that will allow only certain particles through it. Membrane filters are used during membrane filtration in microbiology testing.
Membrane filters are generally microporous plastic films with specific size ratings and extensively used for aggressive solutions. It allows the removal of bacteriostatic or bacterial acetyl agents especially in pharmaceutical, cosmetics, electronics and food beverage industries. The advantage of membrane filtration techniques is that it allows large sample volumes at one time.
Lastly, ensure to select a vessel that maximizes liquid throughput while minimizing "hold-up" volume. All filtration systems retain some amount of liquid as it passes through the membrane, which can result in loss of media and reagents. This retained liquid is called "hold-up" volume. Based on research conducts, Corning filtration systems were shown to have the lowest “hold-up” volume of all major brands, helping you save valuable media and reagents in every filtration.
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