Cell growth performance on various commercial tissue culture polystyrene (TCPS) vessels
Interestingly, no statistically significant difference in the cell confluency of both ECC-1 and MDA-MB-231 in both type of vessels tested.
To further evaluate the cell growth performance, both cell lines were seeded in flask and plate, and incubated for 48 hours in complete culture media at 37oC and 5% CO2. Then, cells were prepared for cell counting using Trypan blue solution.
Total cells per ml of ECC-1 and MDA-MD-231 cell lines in four brands of T25 and T75 cell culture flask.
Total cells per ml of ECC-1 and MDA-MD-231 cell lines in four brands of 6-well culture plate.
ECC-1 cell line
MDA-MB-231 cell line
Cell images of ECC-1 and MDA-MB-231 cell lines in TCPS flask after 72 hours captured at 10x magnification.S
It is important to note that we do not intend to conclude (nor can we conclude) that one particular brand of TCPS is superior to another. Results for other cell types and for specific features of cell responses may differ greatly than results presented here. Additionally, the desired response of cell growth performance and interactions can be unique to a given experimental purpose. In summary, it is important to recognize both the significant and insignificant differences that exist across seemingly similar types of TCPS, which may in turn play significant roles in cueing cell growth performance.
We gratefully acknowledge support from the Cytus Sdn Bhd, University of Malaya for research resources and technology.
At FC-BIOS, we deliver a complete solution for cell culture applications including cultureware, media & reagents, filtrations and equipment. Together with our partner Sorfa Life Science and Corning, we bring the most diverse selection of cell culture products and services available commercially. We did this with researchers like you in mind, to ensure that you have the necessary quality products to further your research goals. We have a specialized team to provide full support and services to customers throughout Malaysia.