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As cell therapy trials advance from pre-clinical research to late-stage trials, cell culture platforms will need to meet increasing demands for cell yield. The Corning® CellCube® system provides a simple, compact, and scalable method for mass culture of attachment-dependent cells. In this webinar, we present data supporting the expansion of two commonly used bioproduction cell lines – HEK293T and Vero. The basic closed system was configured with a peristaltic pump to drive continuous circulation through the CellCube 100-layer module and paired with the Eppendorf BioFlo® 120 controllers with a BioBLU® 3c single-use bioreactor for medium conditioning. The controller simplified oxygenation and pH control of the circulating medium. Medium conditioning in the SUB was sufficient to sustain both HEK293T and Vero cell growth in the CellCube 100-layer module for 5- and 6-day expansions, respectively. Final harvest yields for both HEK293T and Vero cell lines greater than 10 billion cells demonstrate the utility of the CellCube system for mass culture of adherent cell lines with efficient medium usage.