Products Life Science & Cell Culture > Plant Tissue Culture


Plant Tissue Culture Media

The culture medium is one of the most important requirements for plant tissue culture and is one of the fundamental techniques that make plant tissue culture possible. The success of the culture medium is largely dependent on its chemical composition. The composition of each tissue culture may vary for different plants according to their nutritional requirements.

HiMedia offers a wide range of classical as well as crop-specific media with multiple variations. The media are offered in either liquid or powder form to suit the needs of the users. All medias made by HiMedia undergo stringent quality control testing and analysis along with plant tissue culture tests required to maintain consistency and quality.

Customized media are also provided by HiMedia to users with specific requirements and the customization are kept confidential.

Banana

Specialized culture medium made for the propagation of Banana tissues. Usually include addition of vitamins and specific substances to promote banana culture growth such as plant growth regulators, sucrose, casein hydrolysate and others.


Cat No. Description Qty/Pack
PCT076-1L Banana Micropropagation Medium 1 l
PCT075-1L Banana Multiplication Medium 1 l
PCT077-1L Banana Multiplication Medium 1 l
PCT158-1L Banana Rooting Medium 1 l

Orchid

Specialized culture medium made for the propagation of various types of orchid tissues. Usually include addition of vitamins and specific substances to promote banana culture growth such as plant growth regulators, sucrose, casein hydrolysate and others.


Cat No. Description Qty/Pack
PCT062-1L BM-1 Terrestrial Orchid Medium 1 l
PCT152-1L Dendrobium (Orchid) Seed Germination Medium 1 l
PCT106-1L Mitra Orchid Medium 1 l
PCT055-1L Orchid Maintenance Medium 1 l

Potato

Cat No. Description Qty/Pack
PCT090-1L Potato Micropropagation Medium 1 l
PCT132-1L Potato Establishment Medium 1 l
PCT133-1L Potato Multiplication Medium 1 l
PCT134-1L Potato Tuberization Medium 1 l

Strawberry

Cat No. Description Qty/Pack
PCT117-1L Strawberry Multiplication Medium 1 l
PCT117-5L Strawberry Multiplication Medium 5 l
PCT117-25L Strawberry Multiplication Medium 25 l
PCT118-1L Strawberry Rooting Medium 1 l
PCT118-5L Strawberry Rooting Medium 5 l
PCT118-25L Strawberry Rooting Medium 25 l

Tomato

Cat No. Description Qty/Pack
PCT130-1L Tomato Regeneration Medium 1 l
PCT130-5L Tomato Regeneration Medium 5 l
PCT130-25L Tomato Regeneration Medium 25 l
PCT131-1L Tomato Regeneration Medium 1 l
PCT131-5L Tomato Regeneration Medium 5 l
PCT131-25L Tomato Regeneration Medium 25 l


Gelling Agents

Plant tissue culture takes place in a three-dimensional space. Even though callus cells can be grown in liquid media, for the shoot to extend up and roots to penetrate, a semi-solid gel surface is needed. This necessity warrants the use of gelling agents, as they play an important for the growth of the tissue culture.

Even though the primary reason for gelling agents is to provide physical anchorage to the cultures, it also has inherent physical and chemical properties of controlling movement and availability of water and ions in the matrix. This gives it the ability to contribute to the osmotic potential of the medium.

There are a wide variety of gelling agents and the choice of which is determined by the stage of micropropagation and can influence the growth of the culture. HiMedia offers a wide range of gelling agents, all of which is tested for firmness and strength with strength and texture analyzers.

Himedia Gelling Agents

Gelling Agents

Cat No. Description Storage Qty/Pack
PCT0901-500G Agar Powder Store below 30°C 500 g
PCT0907-500G Carrageenan Store below 30°C 500 g
PCT0902-500G CleriGar™ Store below 30°C 500 g
PCT0905-500G CleriGar™ T Store below 30°C 500 g
PCT0903-100G CleriGel™ Ultra Store below 30°C 100 g
PCT0906-100G CleriGel™ Super Store below 30°C 100 g


Plant Growth Regulators

Plant hormones are naturally occurring compounds within plant tissues. These hormones are usually synthesized by certain parts of the plant and are moved through the plant to its endpoint. These hormones regulate the growth and development of a plant.

Through either the fermentation process or chemical synthesis, these chemicals will mimic the action of natural plant hormones. Because these chemicals are able to modify plant growth in certain ways, they have been termed as plant growth regulators.

There are five major classes of plant growth regulators. These include Auxins, Cytokinins, Gibberellins, Polyamines and Abscisic acid. Each of these growth regulators have a specific role and function which can be used to promote various physiological processes in plants.

Plant Tissue Culture 2

Auxins

Cat No. Description Assay Storage Qty/Pack
PCT0841-25G p-Chlorophenoxyacetia acid ≥98% Store below 30°C 25 g
PCT0834-5G Indole-3-acetic acid (IAA) ≥98% Store at 2 – 8°C 5 g
PCT0831-5G Indole-3-propionic acid (IAA) ≥97% Store at 2 – 8°C 5 g
PCT0809-25G α-Naphthaleneacetuc acid (NAA) ≥95% Store at 2 – 8°C 25 g

Cytokinins

Cat No. Description Assay Storage Qty/Pack
PCT0801-10G Adenine sulphate ≥98% Store below 30°C 10 g
PCT0802-1G 6-Benzyladenine (6-BAP) ≥98% Store at 2 – 8°C 1 g
PCT0806-1G Kinetin ≥99% Store at 2 – 8°C 1 g
PCT0820-250MG Thidiazuron (TDZ) ≥92% Store at 2 – 8°C 250 mg

Gibberellins

Cat No. Description Assay Storage Qty/Pack
PCT0830-1G Gibberellic acid (GA3) ≥90% Store at 2 – 8°C 1 g
PCT0830-10G Gibberellic acid (GA3) ≥90% Store at 2 – 8°C 10 g
PCT0830-100G Gibberellic acid (GA3) ≥90% Store at 2 – 8°C 100 g

Others

Cat No. Description Assay Storage Qty/Pack
PCT0837-50MG Ancymidol ≥98% Store at 2 – 8°C 50 mg
PCT0824-1G Chlormequat chloride ≥98% Store below 30°C 1 g
PCT0843-100MG Fluridon ≥99% Store below 30°C 100 mg
PCT0805-100MG (±)-Jasmonic acid ≥85% Store at 2 – 8°C 100 mg


Contamination Control Systems

Microbial contamination is one of the biggest challenges that can occur when using plant tissue cultures. The main issue is that these microorganisms infecting the plant tissue culture will deplete the nutrients found in the medium and subsequently reduce the chances that the plant will survive and grow healthily. Therefore, it is extremely critical to employ an avoidance or prevention strategy in plant tissue culture.

To counter these contamination issues, HiMedia offers a wide range of antimicrobials including antibiotics, antifungals and selection agents. HiMedia also produces proprietary products such as Phytapro™ and antimicrobial supplements that are broad spectrum biocides. These antimicrobials have been specifically designed for use in plant tissue culture.

Himedia Contamination Control Systems

Antibiotics and Antifungals

Cat No. Description Potency Storage Qty/Pack
PCT1107-1G Amoxycillin ≥900μg/mg Store at 2 – 8°C 1 g
PCT1109-1VL Bacitrcin ≥60 U/mg Store at 2 – 8°C  1vl
PCT1142-1G Colistin sulphate >19.000 units/mg Store at 2 – 8°C 1 g
PCT1123-1G Erythromycin ≥850μg/mg Store below 30°C 1 g

Antibiotics Solutions (Sterile Filtered)

Cat No. Description Storage Qty/Pack
PCT1507-20ML Ampicillin solution with 100 mg/ml Ampicillin sodium salt in sterile distilled water Store at -20°C 20 ml
PCT1501-20ML Carbenicillin solution with 100 mg/ml Carbenicillin in sterile distilled water Store at -20°C 20 ml
PCT1504-20ML Kanamycin solution with 50 mg/ml Kanamycin in sterile distilled water Store at 2 – 8°C 20 ml
PCT1506-20ML Neomycin solution with 10 mg/ml Neomycin sulphate in sterile distilled water Store at 2 – 8°C 20 ml

Antimicrobial Supplement

Cat No. Description Storage Qty/Pack
PCT1106-50ML Antimicrobial supplement Store below 30°C 50 ml
PCT1106-100ML Antimicrobial supplement Store below 30°C 100 ml

PhytaPro™

Cat No. Description Storage Qty/Pack
PL047-25ML PhytaPro™ Store below 30°C 25 ml
PL047-100ML PhytaPro™ Store below 30°C 100 ml
PL047-500ML PhytaPro™ Store below 30°C 500 ml

Disinfectants

Cat No. Description Assay Storage Qty/Pack
PCT1322-500ML Benzalkonium chloride 48-55% Store below 30°C 500 ml
PCT1509-500G Calcium hypochlorite ≥98% Store below 30°C 500 g
PCT1511-100ML Hydrogen peroxide min 30% Store at 2 – 8°C 100 ml
PCT1307-10G Silver nitrate ≥99.9% Store below 30°C 10 g


Plant Tissue Culture Tested Chemicals

HiMedia is one of the foremost suppliers of a comprehensive range of plant tissue culture tested chemicals and biochemicals. To verify these chemical’s suitability in their application for plant tissue cultures, the tested chemicals are assessed analytically by HiMedia for chemical parameters. Additional specific plant tissue culture tests are also performed.

HiMedia offers a wide range of chemicals including macroelements, microelements amino acids, carbohydrates, vitamins, and organic supplements that allow users to combine and customize their own formulation, giving huge flexibility to the users.

Consistency and quality is an extremely important criteria to HiMedia. Therefore, all of these chemical products will undergo stringent quality control that ensures its consistency and quality. The testing criteria was developed and defined in-house by HiMedia and the routine testing protocol includes parameters such as appearance, solubility, FTIR, assays, melting range and a application test whereby plant tissue cultures are tested.

Himedia Plant Tissue Culture Tested Chemicals

Adsorbents

Cat No. Description Storage Qty/Pack
PCT1001-100G Activated charcoal powder Store below 30°C 100 g
PCT1004-100G Polyvinylpyrrolidone (PVP 10) Store below 30°C 100 g
PCT1002-100G Polyvinylpyrrolidone (PVP) K-30 Store below 30°C 100 g
PCT1003-100G Polyvinylpolypyrrolidone (PVPP) Store below 30°C 100 g

Amino Acids

Cat No. Description Assay Storage Qty/Pack
PCT0301-25G L-Alanine ≥99% Store below 30°C 25 g
PCT0305-25G L-Cysteine ≥99% Store below 30°C 25 g
PCT0308-25G L-Glutamine ≥99% Store below 30°C 25 g
PCT0312-25G L-Isoleucine ≥99% Store below 30°C 25 g

Buffers

Cat No. Description Assay Storage Qty/Pack
PCT0701-25G MES buffer ≥99% Store below 30°C 25 g

Carbohydrates (Carbon Source)

Cat No. Description Assay Storage Qty/Pack
PCT0608-1G α-Cyclodextrin ≥98% Store below 30°C 1 g
PCT0609-100G β-Cyclodextrin ≥98% Store below 30°C 100 g
PCT0612-500G Dextrose monohydrate ≥99.5% Store below 30°C 500 g
PCT0601-100G D-(-)-Fructose ≥99% Store below 30°C 100 g

Dyes and Stains

Cat No. Description Assay Storage Qty/Pack
PCT1514-10G Alcian blue 8GX ≥99.5% Store below 30°C 10 g
PCT1515-5G Bromocresol purple ≥99.5% Store below 30°C 5 g
PCT1516-5G Bromothymol Blue - Store below 30°C 5 g
PCT1517-25G Methylene blue, trihydrate ≥99.5% Store below 30°C 25 g

Enzymes

Cat No. Description Activity Storage Qty/Pack
PCT1518-5KU Cellulase - Store at 2 – 8°C 5 ku
PCT1531-1G Macerozyme R-10 3000 U/g Store at 2 – 8°C 1 g
PCT1519-5KU Pectinase 8000 – 12000 U/g Store at 2 – 8°C 5 ku
PCT1520-25MG Pectolyase ≥0.3 unit/mg solid Store at 2 – 8°C 25 mg

Indicators

Cat No. Description Assay Storage Qty/Pack
PCT1209-1G Blue tetrazolium chloride 95% Store below 30°C 1 g
PCT1201-500MG BCIP 98% Store at 2 – 8°C 500 mg
PCT1208-100MG X-α-Gal 98% Store at 2 – 8°C 100 mg
PCT1202-100MG X-Gal 98% Store at 2 – 8°C 100 mg

Macro Elements

Cat No. Description Assay Storage Qty/Pack
PCT0001- 500G Ammonium dihydrogen phosphate 98% Store below 30°C 500 g
PCT0017-500G Calcium chloride anhydrous 93% Store below 30°C 500 g
PCT0125-500G Magnesium sulphate anhydrous 99% Store below 30°C 500 g
PCT0009-500G Potassium dihydrogen phosphate 99% Store below 30°C 500 g

Media Dyes

Cat No. Description Storage Qty/Pack
PCT1313-250G Apple green colour Store below 30°C 250 g
PCT1314-250G Blue colour Store below 30°C 250 g
PCT1312-250G Pea green colour Store below 30°C 250 g
PCT1315-250G Yellow colour Store below 30°C 250 g

Micro Elements

Cat No. Description Assay Storage Qty/Pack
PCT0119-25G Ammonium molybdate tetrahydrate ≥99% Store below 30°C 25 g
PCT0102-500G Boric acid ≥99.5% Store below 30°C 500 g
PCT0103-100G Cobalt chloride hexahydrate ≥99% Store below 30°C 100 g
PCT0104-250G Copper (II) suphate petahydrate ≥99.5% Store below 30°C 250 g

Miscellaneous Chemicals

Cat No. Description Assay Storage Qty/Pack
PCT1301-1G Acetosyringone ≥98% Store at 2 – 8°C 1 g
PCT1526-100MG Acetylsalicylic acid ≥98.5% Store below 30°C 100 mg
PCT1528-100G Calcium carbonate ≥98% Store below 30°C 100 g
PCT1326-50G Calcium silicate ≥95% Store below 30°C 50 g

Organic Acids

Cat No. Description Assay Storage Qty/Pack
PCT0501-500G Citric acid anhydrous ≥99.7% Store below 30°C 500 g
PCT0505-25G α-Ketoglutaric acid ≥99% Store at 2 – 8°C 25 g
PCT0502-250G L-(-)-Malic acid ≥96.5% Store below 30°C 250 g
PCT0503-25G Sodium pyruvate ≥99% Store below 30°C 25 g

Organic Supplements

Cat No. Description Storage Qty/Pack
PCT0401-250G Apple powder Store below 30°C 250 g
PCT0402-100G Banana powder Store below 30°C 100 g
PCT0414-100G Coconut water Store at 2 – 8°C 100 g
PCT0406-500G HiVeg™ Peptone No.1 Store below 30°C 500 g

Steroids/Sterols

Cat No. Description Assay Storage Qty/Pack
PCT1521-5MG Brassicasterol ≥98% Store at 2 – 8°C 5 mg
PCT1522-10MG Brassinolide ≥90% Store below 30°C 10 mg
PCT1523-5MG Campesterol ≥99% Store at -20°C 5 mg
PCT1524-10G β Sitosterol ≥99% Store at -20°C 10 g

Vitamins

Cat No. Description Assay Storage Qty/Pack
PCT0207-50G L-Ascorbic acid ≥99% Store below 30°C 50 g
PCT0201-1G D-Biotin ≥98.5% Store at 2 – 8°C 1 g
PCT0202-25G Calcium D-pantothenate ≥98% Store at 2 – 8°C 25 g
PCT0216-1G Cholecalciferol ≥97% Store at 2 – 8°C 1 g