Products > Transplant Diagnostics > HLA Antibody Detection

HLA Antibody Detection

Bead-based Multiplexing Assay for PRA Screening

LABScreen reagents are powered by Luminex® xMAP® technology, a microbead platform used to deliver multiplex antibody assays in high-PRA patients. This single antigen-bead based assay allows for a precise determination of antibody profiles against HLA, MICA or HNA. The proven reliability of LABScreen’s consistency, high sensitivity and robustness for PRA screening has gained rapid momentum in the transplant community.

Key Features

Proven Accuracy

  • Distinguishes HLA Class I and Class II antibodies
  • Contains a purified single antigen or a defined pool of HLA antigens, including rare alleles
  • Eliminates false positive reactions due to non-HLA antibodies or auto antibodies
  • Single antigen assay identifies negative or safe antigens, even for high PRA patients
  • Detects IgG antibodies

Premier Automation

  • Based on Luminex® xMAP® technology
  • Provides software-driven data acquisition

Maximum Consistency

  • High reproducibility
  • Delivers reaction-to-reaction consistency

LABScreen® Single Antigen

LABScreen® Single Antigen

Donor Specific Antibody Monitoring — A New Standard of Care

LABScreen Single Antigen assays leverage Luminex® bead-based multiplexing technology for monitoring Donor Specific Antibodies (DSA) in high-PRA patients for both pre- and post-transplant applications. This single antigen-bead based assay allows for a precise determination of antibody profiles against HLA, MICA or HNA. The proven reliability of LABScreen’s consistency, high sensitivity and robustness for PRA screening has gained rapid momentum in the transplant community.

In high PRA patients, antibody reactive to one or more dominant epitopes can mask the presence of additional antibody specificities. Conventional clarification methods may not be effective in these cases. LABScreen Single Antigen is designed to screen HLA Class I and Class II antibodies that are reactive to one or more dominant epitopes providing clinicians a comprehensive assay to evaluate antigens found frequently in the population.

SSA

 


Cat No. Product Target Number of Test
LS1A04 LABScreen Single Antigen HLA Class I – Combi HLA-A, HLA-B, HLA-C 25
LS2A01 LABScreen Single Antigen HLA Class II – Group 1 HLA-DRB, HLA-DQB 25


LABScreen® PRA

Determines percent PRA and identifies antibody specificities using a panel of color-coded microbeads coated with HLA antigens purified from different cells. Class I and Class II assays may be tested separately or together. Utilizes the LABScan™ 100 or LABScan3D™ for analysis of up to 100 or 500 bead regions, respectively, in a single test.

FEATURES & BENEFITS

  • 96 samples can be tested sequentially using the X-Y platform
  • Identifies negative or safe antigens, even for high PRA patients
  • Detects IgG antibodies
  • Ideal for low volume and high throughput laboratories

pra


Cat No. Product Format Number of Test
LS1PRA LABScreen PRA Class I 55 – Class I antigen panel 25
LS2PRA LABScreen PRA Class II 35 – Class II antigen panel 25
LS12PRA LABScreen PRA Class I and Class II 55 – Class I and 32 – Class II antigens 25


LABScreen Mixed

A screening test dedicated to detecting antibodies agains HLA Class I and Class II in addition to MHC Class I related chain A (MICA), another factor shown to cause transplant rejection. With its ease of use, processing up to 96 samples simultaneously, LABScreen Mixed, is well-suited for monthly patient antibody screening in both low and high throughput laboratories.

FEATURES & BENEFITS

  • Identifies HLA Class I/II, and MICA
  • Single tube protocol
  • Suited for low and high throughput laboratories

mixed


Cat No. Product Regulatory Format Number of Test
LSM12 LABScreen™ Mixed For In Vitro Diagnostic Use Class I/II combined, plus MICA gene 100


LABScreen MICA

Some studies suggest a relationship between MICA antibodies and solid organ transplant rejection where no HLA antibodies are detected. LABScreen MICA was created for HLA antibody screening and identification of MHC class I related chain A (MICA) antibodies. A valuable tool for specificity assignment, each recombinant MICA (rMICA) antigen is coated on a separate bead population. Advanced custom analytic capabilities are available with the combined use of our HLA Fusion™ Software and multiplex analyzer, LABScan™ 100 or LABScan3D™.

FEATURES & BENEFITS

  • Quickly identifies Class I MICA antibodies in high PRA patients
  • Identifies negative or safe antigens, even for high PRA patients
  • Confirms specificity by replacing dilution and absorption as strategies
  • Clarifies ambiguous results
  • Clarifies analysis of antibodies reactive to one or several dominant epitopes

mica


Cat No. Product Regulatory Format Number of Test
LSMICA001 LABScreen MICA Single Antigen – Group 1 For In Vitro Diagnostic Use 11-MICA antigen panel 25


LABScreen® Single Antigen Supplement

LABScreen Single Antigen product family is used to help advance DSA monitoring by screening antibodies against HLA antigens found in higher frequencies among certain regional populations. When used in conjunction with LABScreen Single Antigen, the comprehensive panel of common and rare HLA antigens accurately defines HLA antibodies in complex sera from around the world.

FEATURES & BENEFITS

  • 96 samples can be tested sequentially using the X-Y platform
  • Identifies negative or safe antigens, even for high PRA patients
  • Detects IgG antibodies
  • Ideal for low volume and high throughput laboratories

single antigen supplement


Cat No. Product Format Number of Test
LS1ASP01 LABScreen Single Antigen Class I – Supplement (Group1) HLA-A, B, C 25


LABScreen® Ancillary Reagents

Ancillary products for use with LABScreen such as control beads and serum, Adsorb Out, Quantiplex Beads, wash buffer, and other reagents.

Usage:

Negative control serum used as an indicator of the non-specific background signal of each LABScreen and LABScreen Multi bead when reacting with a serum sample that does not contain any anti-HLA Class I or Class II antibody.

Ancilary reagents


Cat No. Product Format Number of Test
LS-NC LABScreen Negative Control Serum Control reagent 20